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| García-Muse, T., Aguilera, A. Transcription–replication conflicts: how they occur and how they are resolved. Nat Rev Mol Cell Biol 17, 553–563 (2016). |
What Happens When Replication Fork and Transcription Machinery Clash?
Introduction
When the replication fork and transcription machinery come into contact, it can result in DNA damage and mutations, leading to genomic instability and potential cell death. However, cells have developed mechanisms to prevent these collisions and resolve them if they occur.
Replication and Transcription Processes
Briefly explain how DNA replication and transcription occur. Discuss the movement of both processes along the DNA and how collisions can occur.
Consequences of Replication-Transcription Clashes
Explain the DNA damage and mutations that can occur as a result of these collisions. Discuss the potential consequences, such as genomic instability and cell death.
Mechanisms to Prevent and Resolve Collisions
Describe how cells use chromatin structure to compartmentalize DNA into distinct domains for replication and transcription. Discuss the specific proteins that can resolve replication-transcription conflicts and facilitate the resumption of both processes without causing DNA damage.
Conclusion
While collisions between replication and transcription machinery can be detrimental to DNA, cells have evolved mechanisms to prevent and resolve these conflicts. Understanding these mechanisms can provide insight into the processes that maintain genomic stability and prevent mutations.
